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It’s in the settings for Backup and Sync. If you’re on a Windows machine, the icon for Backup and Sync is in the lower left of your screen – in the System tray – right click that and choose preferences. You don’t actually specify individual files to back up – you specify folders. So you would need to uncheck the folder – or just move the file out of the watched folder.
This video might help – it starts with installing Backup and Sync, but then it does show the settings.